Synchrotron-based small-angle X-ray scattering of proteins in solution
نویسندگان
چکیده
منابع مشابه
The Solution of the Eigenvector Problem in Synchrotron Radiation Based Anomalous Small-Angle X-Ray Scattering
In the last three decades Synchrotron radiation became an indispensable experimental tool for chemical and structural analysis of nano-scaled properties in solid state physics, chemistry, materials science and life science thereby rendering the explanation of the macroscopic behavior of the materials and systems under investigation. Especially the techniques known as Anomalous Small-Angle X-ray...
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Synchrotron small angle x-ray scattering (SAXS) has been applied to the structural study of several biological systems, including the nitrogenase complex, the heat shock cognate protein (hsc70), and lysozyme folding. The structural information revealed from the SAXS experiments is complementary to information obtained by other physical and biochemical methods, and adds to our knowledge and unde...
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ABSTRACT Background: Coherent scattering leads to diffraction effects and especially constructive interferences. Theseinterferences carry some information about the molecular structure of the tissue. As breast cancer isthe most widespread cancer in women, this project evaluated the application of small angleX-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues....
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We constructed chimeric proteins that consist of two green fluorescent protein variants, EBFP and EGFP, connected by flexible linkers, (GGGGS)n (n = 3 approximately 4), and helical linkers, (EAAAK)n (n = 2 approximately 5). The conformations of the chimeric proteins with the various linkers were evaluated using small-angle X-ray scattering (SAXS). The SAXS experiments showed that introducing th...
متن کاملAccuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
One of the most important overall parameters, which can be derived from smallangle X-ray scattering (SAXS) experiments on macromolecular solutions is the molecular mass (MM) of the solute. In particular, for a monodisperse protein solution, MM of the solute is calculated from the extrapolated scattering intensity at zero angle I(0). Assessing MM by SAXS provides valuable information about the o...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2014
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2014.116